This contribution examines the deployment of knowledge and practices in regenerative agriculture from a gender perspective in Andean and Andean-Amazonian transition ecosystems in Colombia, Ecuador, and Peru. This approach makes visible often-overlooked contributions within food systems and value chains; that can be understood as concrete possibilities for Ecosystem-based Adaptation (EbA), extending beyond economic value frameworks toward an integrated approach centered on the reproduction of life. This perspective includes reducing climate risk in Latin America and enabling more equitable transformations. Using qualitative content analysis, this study aims to contribute to theoretical and methodological reflections on gender perspectives in the literature by integrating life reproduction and care economy approaches within food systems. Additionally, the research synthesizes scientific and technical findings on women’s contributions to these systems. Based on our findings, we will provide comprehensive insights for the Futuring Landscapes program on addressing gender within food value chains. This includes concrete examples of the complex relationships between gender and the territories where agrobiodiversity-based food is produced, processed, marketed, and consumed.