District Swabi, with its typical dried extension of ecoregions of Himalaya subtropical pine forest, Hindukush and Baluchistan xeric woodland that includes sandy grasslands, subtropical, dry sub-temperate Hindu Kush- Himalaya mountains and ecotone microhabitats with distinguish River Indus divided Himalaya-Hindukush mountains, is reported here one of the Pakistan biodiversity hotspot for spider diversity. A total of 67 spider species belonging to 37 genera and 30 families were identified through several years of extensive survey work. The distribution records of 50 species have been updated. The family Salticidae is most diverse with 32 species belonging to 22 genera followed by Araneidae with 23 species in 17 genera. The guild structure analysis revealed with majority Stalker, web-weaver, Ambusher, scattered line weaver, foliage hunter, funnel web builder respectively, and include habitat specialists that favor specific microhabitats. Some of these habitat specialists may be affected by alterations on ecotone and river Indus environment due to anthropogenic and climate change of drought and flood.